The PCR Song
There was a time when to amplify DNA,
You had to grow tons and tons of tiny cells.
Then along came a guy named Dr. Kary Mullis,
Said you can amplify in vitro just as well.
Just mix your template with a buffer and some primers,
Nucleotides and polymerases, too.
Denaturing, annealing, and extending,
Well it’s amazing what heating and cooling and heating will do.
PCR - when you need to detect mutation
PCR - when you need to recombine
PCR - when you need to find out who the daddy is
PCR - when you need to solve a crime
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